RT-PCR (A) and Western blot analysis (B) of inductive expression of hLF transgene.
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(A) Lane 1: non-transgenic mammary epithelial cells, lane 2: transgenic fibroblast cells, lane 3: transfected mammary epithelial cells, lane 4: vector pBC1-hLF-Neo-IRES2-EGFP as positive control. GAPDH was used as a reference gene. (B). Lane 1: Recombinant hLF (42 kDa) was detected in the supernatants from transfected mammary epithelial cells cultured in the inductive medium, lane 2: No signals were present in the supernatants from transfected fibroblasts, lane 3: No signals were present in the supernatants from transfected epithelial cells without inductive medium, lane 4: no signals were present in the supernatants from normal epithelial cells with inductive medium.
创建时间：
2016-02-23
