Secretome analysis in CAF-dependent conditioned mediums with FaDu cells

We co-cultured primary cultured CAF (CAF-P), which promotes progression more actively, and CAF (CAF-D), which have less cancer-promoting properties, with OSCC cells, and analyzed the differential secretion through antibody microarray. To evaluate the differential effect of the two CAF cell types on cisplatin efficacy in OSCC cells, we co-cultured FaDu spheroids (bottom well) with OSCC cells, CAF-P or CAF-D for 48 h in transwells. Antibody arrays were performed for each CM.