snRNAseq of mouse thick ascending limb cells

Kidney thick ascending limb (TAL) cells display a remarkable amount of regional and functional heterogeneity. We applied single-nucleus RNA-seq to map the molecular heterogeneity of enriched Slc12a1+ cells. Overall design: Kidney thick ascending limb (TAL) cells from Slc12a1-CreERT2-INTACT mice were isolated by Fluorescence-activated nuclei sorting (FANS) according to the presence or absence of GFP signal and analyzed using snRNAseq.