Tissue-specific alteration of gene expression and function by RU486 and the GeneSwitch system

The drug-inducible GeneSwitch (GS)-Gal4 transgene expression system is routinely used in Drosophila aging and behavioral studies to avoid the confounding effects related to genetic background mutations. Here, we report transcriptional and functional defects that are induced by RU486 in a stock- and tissue-dependent manner, such as defects in flight and mitochondrial gene expression. Our findings indicate that side effects induced by RU486 need to be considered when interpreting the observed phenotypes, and proper controls, consisting of ethanol-treated GS-Gal4 flies and RU486-induced expression of mock transgenes, should always be included

药物诱导型GeneSwitch（GS）-Gal4转基因表达系统是果蝇衰老与行为学研究中的常规技术手段，用于规避遗传背景突变所引发的混杂效应。本文报道了RU486以品系和组织依赖的方式诱导产生的转录与功能缺陷，具体包括飞行能力缺陷及线粒体基因表达异常。本研究结果表明，在解读观测到的表型时，需充分考量RU486所诱导的副作用；且实验应始终设置恰当的对照组别，包括经乙醇处理的GS-Gal4果蝇，以及经RU486诱导的空载转基因表达样本。

搜集汇总

背景与挑战

背景概述

该数据集基于果蝇模型，利用RNA-Seq技术研究RU486药物和GeneSwitch系统对基因表达和功能的组织特异性影响。研究发现RU486可能引起飞行缺陷和线粒体基因表达等副作用，这些影响因组织和品系而异，强调了在实验设计中纳入适当对照的重要性。数据集包括胸部和头部器官的转录组数据，旨在为衰老和行为研究提供参考。