Measurements of peptidase and glucosidase activities in large volume mesocosm incubations on RV/Endeavor EN584, July 2016 (Patterns of activities project)

<p>Fluorescence was measured over 24-48 hours incubation time with a plate reader (TECAN spectrafluor plus; 360 nm excitation, 460 emission), with time points taken every 4-6 hours. Hydrolysis rates were calculated from the rate of increase of fluorescence in the incubation over time relative to a set of standards of known concentration of fluorophore. Scripts to calculate hydrolysis rates and produce the figures shown here are available in the associated Github repository [Hoarfrost, 2017].</p> <p>See Niskin Bottle and Cast List EN584 to link specific casts and bottles to each experiment: BCO-DMO dataset 717427&nbsp;(see related datasets).</p>采用TECAN SpectraFluor Plus型酶标仪（plate reader），以360 nm为激发波长、460 nm为发射波长，对24至48小时孵育过程中的荧光信号进行检测，每4至6小时采集一次时间点数据。水解速率通过孵育体系中荧光强度随时间的增长速率，结合一系列已知浓度的荧光团（fluorophore）标准品计算得到。用于计算水解速率及生成本文附图的代码脚本，可在关联的GitHub代码仓库[Hoarfrost, 2017]中获取。 可参考尼斯金采水器（Niskin Bottle）与EN584站位投放列表（Cast List EN584），将特定采样站位与采水器对应至各项实验：BCO-DMO数据集717427（详见相关数据集）。