RNA sequencing of total RNA from spinal cord total lysates of the PS19 tauopathy mouse model.

RNA sequencing was perfomed to compare transcriptomic profiles of PS19 and control mice at four and nine months. Total RNA was isolated from the spinal cord of PS19 and control mice at four and nine months. PolyA selected RNA was then sequenced using an Illumina HiSeq 6000 with 150b bp paired-end stranded reads at ~20 million reads per sample. The founder PS19 mice were obtained from the Jackson Laboratory (IMSR_JAX:008169). Heterozygotic offspring were bred and maintained at Baylor College of Medicine. Wild-type littermates were used as controls.本研究采用RNA测序技术，对比4月龄与9月龄PS19小鼠及对照小鼠的转录组谱。研究人员分别提取4月龄及9月龄PS19小鼠与对照小鼠脊髓组织的总RNA，随后对聚腺苷酸筛选RNA（polyA selected RNA）进行测序：使用Illumina HiSeq 6000平台，采用150 bp双端链特异性读长，每个样本的测序读段数约为2000万。创始株PS19小鼠购自杰克逊实验室（Jackson Laboratory，IMSR_JAX:008169），其杂合子后代在贝勒医学院（Baylor College of Medicine）内繁育并保种，本研究以同窝野生型小鼠作为对照。