Single-cell RNA sequencing of non-hematopoietic cells from mouse thymus spanning embryonic and adult stages

Here we constructed a single-cell transcriptional landscape of non-hematopoietic cells from mouse thymus spanning embryonic and adult stages, producing transcriptomes of 30,959 TECs. We resolved transcriptional heterogeneity of developing TECs, and highlighted the molecular nature of early TEC lineage determination and cortico-medullary thymic epithelial cell lineage divergency. We purified non-hematopoietic cells from thymi at embryonic day 11.5 (E11.5, n=30) and E12.5 (n=30), E13.5 (n=23), E14.5 (n=26), E15.5 (n=25), E16.5 (n=26), newborn (n=14), 5-week old (n=4) from C57BL/6 mice. And we performed single-cell transcriptomics by droplet-based 10X Genomics.