Adaptive evolution targets a piRNA precursor transcription network

File "cuff cDNA sequences.docx" contains cloned cuff cDNA sequences from Drosophila melanogaster and Drosophila simulans used to make transgenes. Files "nG_Cuff_proteomics" and "rhiP_Cuff_proteomics" are scaffold files, which contains mass spectrometry protein spectrum counts for Cuff immuno-precipitates. Cuff proteins are either driven by rhi promoter (rhiP) or UASp promoter under nos-Gal4 driver (nG). These files can be opened by Scaffold Protein Spectra Viewer by Proteome Software.文件"cuff cDNA sequences.docx"收录了源自黑腹果蝇（Drosophila melanogaster）与拟暗果蝇（Drosophila simulans）的克隆型cuff cDNA序列，此类序列可用于制备转基因构件。 文件"nG_Cuff_proteomics"与"rhiP_Cuff_proteomics"为Scaffold格式文件，其中包含针对Cuff免疫沉淀产物的质谱蛋白质谱计数数据。 Cuff蛋白的表达可通过两种启动子驱动：其一为rhi启动子（rhiP），其二为受nos-Gal4驱动系统（nG）调控的UASp启动子（UASp promoter）。 此类文件可通过Proteome Software公司开发的Scaffold蛋白质谱查看器（Scaffold Protein Spectra Viewer）打开。