1Symbols used: “ = seconds, ‘ = minutes.2Two separate reactions were carried out with 2 different reverse primers (CYA781Ra & CYA781Rb) according to [48]. After amplification, samples were pooled, mix

Figshare2015-12-02 更新30

Additional file 10 of The complete mitochondrial genome of the citrus red mite Panonychus citri (Acari: Tetranychidae): high genome rearrangement and extremely truncated tRNAs

线粒体测序

分子生物学实验方法

Additional file 10: Primer sequences and PCR conditions used to amplify the mitochondrial genome of Panonychus citri. (XLS 24 KB)

Figshare2010-10-23 更新30

Primer sequences and cycling conditions used in real-time PCR.

定量PCR引物设计

分子生物学实验方法

Primer sequences and cycling conditions used in real-time PCR.

NIAID Data Ecosystem30

Program for the site-directed mutagenesis PCR.

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