Generation of Slc22a6-CreERT2 knock-in mice.

(A) Schematic diagram of the generation of Slc22a6-CreERT2 knock-in mice. The exon-intron structure of the mouse Slc22a6 locus is shown at the top. The targeting vector has a 4.3 kb 5′ arm that locates immediately upstream of the Slc22a6 gene ATG, the CreERT2-pA coding sequence, a PGK-hygromycin selection cassette flanked by FRT sites (green triangles) and a 5.3 kb 3′ arm that extends to intron 6. (B) Southern blots show a control (wild-type) and 4 correctly targeted ES cell clones (77, 79, 92, 185) that give expected hybridisation patterns. Clone 142 is incorrectly targeted. The location of 5’, 3’ and internal probes as well as expected band lengths are indicated in the diagrams on the right.