Retinal Leukocyte Infiltration Assay.

A) Mice were injected intraperitoneally with either PBS, Pam3CSK4 (25 µg per animal, in PBS), or dipeptide-CEP (400 µg per animal, in PBS) and the retinas were analyzed 8 hours later. Retinal infiltration by neutrophils (Gr1+ cells) or macrophages (F4/80+ cells) was assessed by immunostaining with the respective markers and quantitated with Axiovision, as described in Materials and Methods. Statistical analysis was performed using the Student t-test. Only statistically significant differences are indicated in the graph. B) Shown are representative images of the experiment in Figure 3A. Arrows indicate examples of macrophages or neutrophils in the corresponding images. CEP, Dipeptide-CEP; Pam3, Pam3CSK4.