COS markers showing polymorphic (≥2 bp) PCR amplicons between wheat and <i>Aegilops</i> species are considered as suitable for the marker-assisted introgression into hexaploid wheat of the U and M genome chromosomes from <i>Ae. umbellulata</i> (UU) and <i>Ae. comosa</i> (MM) and from <i>Ae. biuncia

The size (in bp) of the chromosome-specific loci is shown in brackets. Asterisks indicate the loci with predicted chromosomal location when the PCR amplicon was specific for the U or M genomes and could be determined unambiguously in at least one Aegilops species (in the diploid progenitor, or in Ae. biuncialis or Ae. geniculata) and when the highest PCR product yield in the other two species was detected in the subgenomic DNA sample containing the same chromosome. *Loci with predicted chromosomal location.