RNA-seq analysis of whole liver derived from endothelial specific TAK1 conditional knockout mice.

In this study we show that TGFß-activated kinase 1 (TAK1), is crucial for ensuring EC survival on TNFa stimulation. EC-specific TAK1-knockout in adult mice resulted in rapid death of the animal due to destruction of intestinal and liver blood vessels. TNFa from myeloid cells responding to intestinal microbiota was responsible for EC death in the intestine and liver. Our results illuminate mechanisms ensuring survival of intestinal and liver EC under physiological conditions Overall design: Liver mRNA expression profiles derived from VEcadCreERT2/TAK1flox/flox mice injected by tamoxifen were generated by using HiSeq 2500 in triplicate.