The impact of insecticide resistance on Culex pipiens immunity

Because of their role as vectors of diseases, the evolution of insecticide resistance in mosquitoes has been intensively investigated. Insecticide resistance is associated to a wide range of pleiotropic effects on several key life history traits of mosquitoes such as longevity and behavior. However, despite its potential implications in pathogen transmission, the effects of insecticide resistance on mosquito immunity have received little, if any, attention. Here, we investigate the impact of insecticide resistance in Culex pipiens, an epidemiologically important vector of a wide array of pathogens. Using both isogenic laboratory strains and field-caught mosquitoes, we investigate the impact of two main insecticide resistance mechanisms (metabolic detoxification and target site modification) on the relative transcription of several genes involved in the immune response to pathogens, at both their constitutive and inducible levels. Our results show a discrepancy between the isogenic labor...鉴于蚊虫作为疾病传播媒介的重要作用，学界针对蚊虫抗药性（insecticide resistance）的演化开展了大量深入研究。蚊虫抗药性与其诸多关键生活史性状（如寿命、行为）存在广泛的多效性关联。然而，尽管抗药性可能对病原体传播产生潜在影响，但学界对抗药性如何影响蚊虫免疫功能的研究却寥寥无几，即便有也极为有限。本研究以尖音库蚊（Culex pipiens）为研究对象——该物种是多种病原体的流行病学重要传播媒介——探讨抗药性的影响。本研究同时采用实验室同基因品系与野外捕获的蚊虫，针对两种主要抗药性机制（代谢解毒作用与靶标位点修饰），分别在组成型与诱导型表达水平下，探究其对病原体免疫应答相关基因相对转录水平的影响。本研究结果显示，同基因实验室品系与……