SIPA1L1/SPAR1 Interacts with the Neurabin Family of Proteins and is Involved in GPCR Signaling. Matsuura et al. Raw data for super-resolution colocalization analyses.

Super-resolution microscopic images stained for SIPA1L1 and its candidate interacting-proteins in the neuropil region of layer V cerebral cortex or hippocampal CA1 area were acquired using Olympus SD-OSR, a confocal-based spinning disk super-resolution microscope, which implements structured illumination microscopy (SIM). Four serial Z-stack images (200-nm step size) of 1024 x 1024 pixels (41 x 41 um2)/image were used for colocalization analyses. w1 files are for SIPA1L1 and w2 files for others in all folders with the exception of drebrin and actinin where SIPA1L1 is w2.本数据集所包含的超分辨率显微图像，是针对大脑皮层第五层神经毡区域或海马CA1区中SIPA1L1及其候选互作蛋白进行染色后采集所得，成像设备为奥林巴斯SD-OSR（Olympus SD-OSR）共聚焦转盘式超分辨率显微镜，该设备搭载结构照明显微镜（structured illumination microscopy, SIM）技术。本次共定位分析采用4组连续Z堆叠序列图像，单张图像分辨率为1024×1024像素，对应成像视野面积为41×41 μm²，Z轴采样步长为200 nm。在所有文件夹中，w1格式文件对应SIPA1L1的染色图像，w2格式文件对应其余靶标蛋白的染色图像；但在针对树突蛋白（drebrin）与辅肌动蛋白（actinin）的文件夹中，SIPA1L1的染色图像对应w2格式文件。