Kellet's whelk genome and transcriptome assembly

Understanding genomic characteristics of non-model organisms can help bridge gaps in ecology and evolutionary sciences, but lack of a reference genome and transcriptome for these species challenges their study. We advance this goal by conducting the first full genome and transcriptome sequence assembly and analysis of the non-model organism Kellet’s whelk, Kelletia kelletii, a marine gastropod and fisheries species exhibiting a northern range expansion along the US west coast that is potentially driven by climate change. We used a combination of Oxford Nanopore Technologies, PacBio, and Illumina platforms for sequencing, and integrated a set of bioinformatic pipelines to create a comprehensive and contiguous de novo genome assembly. Our results represent the most complete and continuous documented genome among the Buccinoidea superfamily to date. Genome validation revealed its relatively high completeness with low missing metazoan BUSCOs, and an average coverage of ~70x for all contigs,..., , , # Kellet's whelk genome and transcriptome assembly ## Description of the data and file structure See for methods used for the assembly and analysis of the genome and transcriptome. **Files** Genome assembly: Scaffolds_pass1.fasta Genome annotation: transcripts.fasta.transdecoder.genome.gff3 Transcriptome assembly: final_transcriptome.fasta ## Sharing/Access information Data was derived from the following sources: * All raw sequence data, including the PacBio sequel 2, Nanopore MinION, and Illumina NovaSeq DNA sequencing, as well as the Illumina NovaSeq RNA sequencing, are deposited in NCBI Sequence Read Archive (SRA) under PRJNA999368: and PRJNA1000198: . ## Code/Software See methods and commands used at解析非模式生物（non-model organism）的基因组特征，有助于填补生态学与进化科学领域的研究空白，但此类物种缺乏参考基因组与转录组，始终是其相关研究的核心障碍。本研究针对非模式生物凯莱特峨螺（Kellet's whelk，*Kelletia kelletii*）开展了首例全基因组与转录组序列组装及分析工作，以此推进相关研究目标。该物种为海洋腹足类渔业物种，其分布范围沿美国西海岸向北扩张，这一现象可能由气候变化驱动。 本研究结合牛津纳米孔技术（Oxford Nanopore Technologies）、PacBio与Illumina测序平台进行测序，并整合多套生物信息学分析流程，构建了高质量且连续的从头（de novo）基因组组装。本研究所得组装结果，是当前皱唇螺总科（Buccinoidea）中已报道的最完整、连续性最佳的基因组。基因组验证结果显示，该组装的完整性较高，后生动物BUSCO（Benchmarking Universal Single-Copy Orthologs）缺失率较低，所有重叠群（contig）的平均覆盖度约为70×。 # 凯莱特峨螺基因组与转录组组装 ## 数据与文件结构说明 有关基因组与转录组组装及分析的方法，请参见相关文档。 ### 文件列表 基因组组装文件：Scaffolds_pass1.fasta 基因组注释文件：transcripts.fasta.transdecoder.genome.gff3 转录组组装文件：final_transcriptome.fasta ## 数据共享与获取说明 本数据集源自以下资源： * 所有原始测序数据，包括PacBio Sequel 2、Nanopore MinION与Illumina NovaSeq的DNA测序数据，以及Illumina NovaSeq的RNA测序数据，已提交至NCBI序列读取档案库（Sequence Read Archive, SRA），收录编号分别为PRJNA999368与PRJNA1000198。 ## 代码与软件说明 相关使用的方法与代码命令，请参见相关文档。