Data from: Spatial and quantitative datasets of the pancreatic β-cell mass distribution in lean and obese mice

A detailed understanding of pancreatic β-cell mass distribution is a key element to fully appreciate the pathophysiology of models of diabetes and metabolic stress. Commonly, such assessments have been performed by stereological approaches that rely on the extrapolation of two-dimensional data and provide very limited topological information. We present ex vivo optical tomographic data sets of the full β-cell mass distribution in cohorts of obese ob/ob mice and their lean controls, together with information about individual islet β-cell volumes, their three-dimensional coordinates and shape throughout the volume of the pancreas between 4 and 52 weeks of age. These data sets offer the currently most comprehensive public record of the β-cell mass distribution in the mouse. As such, they may serve as a quantitative and topological reference for the planning of a variety of in vivo or ex vivo experiments including computational modelling and statistical analyses. By shedding light on intra- and inter-lobular variations in β-cell mass distribution, they further provide a powerful tool for the planning of stereological sampling assessments.全面了解胰腺β细胞质量（pancreatic β-cell mass）分布，是充分解析糖尿病模型与代谢应激相关病理生理学机制的核心要素。既往此类评估多采用体视学方法（stereological approaches），该类方法依赖二维数据外推，仅能提供极为有限的拓扑学信息。本研究发布了肥胖ob/ob小鼠（ob/ob mice）及其瘦型对照队列的完整胰腺β细胞质量分布离体光学断层扫描（ex vivo optical tomographic）数据集，同时涵盖4至52周龄小鼠全胰腺体积内的单个胰岛β细胞（islet β-cell）体积、三维坐标与形态特征信息。该数据集为小鼠胰腺β细胞质量分布提供了当前最为全面的公开记录。据此，其可作为包括计算建模与统计分析在内的多种体内、离体实验规划的定量与拓扑学参考基准。同时，通过阐明β细胞质量分布的小叶内与小叶间变异，本数据集还可为体视学抽样评估的规划提供强有力的工具支撑。