Cellular composition of murine mitral valves during autoimmune valvular carditis

To characterize the cell types that compose the mitral valve during the development of autoimmune valvular carditis, we utilized scRNA sequencing. Live cells from inflamed valves of K/B.g7 mice or uninflamed B.g7 controls were collected and analyzed at 3-, 8-, and 25 weeks of age. Overall design: Live single cells were sorted from digested mitral valves using fluorescence-activated cell sorting (FACS) and analyzed using scRNA sequencing.