hu.MAP

Proteins interact with each other and organize themselves into macromolecular machines (ie. complexes) to carry out essential functions of the cell. We have a good understanding of a few complexes such as the proteasome and the ribosome but currently we have an incomplete view of all protein complexes as well as their functions. The hu.MAP attempts to address this lack of understanding by integrating several large scale protein interaction datasets to obtain the most comprehensive view of protein complexes. Specifically, we integrated two large scale affinity purification mass spectrometry (AP/MS) datasets from Bioplex and Hein et al. with our dataset of large scale biochemical fractionations (Wan et al) and produced a complex map with over 4k complexes.蛋白质彼此相互作用并组装为大分子机器（即复合物），以执行细胞的核心生理功能。目前我们对少数复合物（如蛋白酶体与核糖体）已有较为充分的认知，但对所有蛋白质复合物及其功能的认知仍不完整。hu.MAP数据集旨在弥补这一认知缺口，通过整合多组大规模蛋白质相互作用数据集，以获取最为全面的蛋白质复合物图谱。具体而言，本研究整合了来自Bioplex与海因等人（Hein et al.）的两组大规模亲和纯化质谱（affinity purification mass spectrometry, AP/MS）数据集，以及本团队的大规模生化分级分离数据集（万等人，Wan et al.），最终构建了包含逾4000个复合物的蛋白质复合物图谱。

搜集汇总

背景与挑战

背景概述

hu.MAP是一个人类蛋白质复合物图谱项目，旨在通过整合多个大规模蛋白质相互作用数据集，如亲和纯化质谱和生化分馏数据，来提供全面的蛋白质复合物视图。该项目已识别出超过4000个复合物，以弥补当前对蛋白质复合物及其功能理解的不完整性。