Mouse Gsr-null liver transcriptome

Genetic disruption of Gsr in mouse elicits only subtle changes in the liver transcriptome. Transcriptome analysis of Gsr-null livers was performed to further our understanding of pathways that may compensate for loss of Gsr, one of the two NADPH-dependent cytosolic disulfide reductases. Livers were harvested from male wild-type or Gsr-null 15-20 week old mice (n = 5, WT; n = 6, Gsr-null) between 2:00 p.m. and 4:00 p.m. Liver RNA was purified and processed for microarray analysis.