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Sequencing of ShCAST RNA-guided in vitro transposition products

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NIAID Data Ecosystem2026-03-12 收录
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Total DNA sequencing of pools of in vitro transposition products in plasmid (pTarget_CAST) using the ShCAST Tn7-CRISPR-Cas transposition system. Investigating role of TnsC ATPase in target-site selection through use of non-hydrolyzable ATP analog and mutant alleles. Mechanistic insights are relevant to genome editing applications.

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2021-06-14
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