Gene expression profile at single cell level of CD11b+ cells from E18.5 fetal brain

Microglia (MG), the brain-resident macrophages, play key roles in the development and maintenance of brain circuits throughout life.Our previous data demonstrated a correlation between maternal-derived GZMB and the activation of embryonic microglia cells, as well as behavioral abnormalities in offspring. Here, we employ single-cell sequencing technology to analyze the heterogeneity of embryonic microglia cells induced by maternal immune activation, including the role of GZMB in this process Overall design: We isolated fetal brains from three groups of pregnant mice (E18.5): Ncr1-cre+Saline, Ncr1-cre+polyIC, and Gzmb-cko+polyIC. Subsequently, we isolated CD11b+ cells using MACS from these samples for single-cell sequencing analysis.