Aim: To evaluate the accuracy of two PCR-based techniques for detecting SARS-CoV-2 variant Alpha (B.1.1.7). Materials & methods: A multicenter prospective cohort with 1137 positive specimens from
aTen parallel Amp-PCR reactions using a JCV-standard containing 180 copies/µl. bTen parallel PCR only-reactions using a JCV-standard containing 180 copies/µl.
We conducted a multicenter evaluation of commercial and in-house PCR methods for the detection of enteroviruses. Three coded panels of test and control RNA samples, artificial clinical specimens, and
Rhizobium leguminosarum symbiovar trifolii (Rlt) nodule samples collected from Store Heddinge, Denmark. These samples were used to validate a metabarcoding method using amplicons with unique molecular
a Proportion of samples positive in the pan-Plasmodium screening of field samples (N = 2977).b The p value, kappa value, sensitivity and specificity were calculated by comparing individual PCRs with t