Guanylyl cyclase was not involved in the inhibitory effects of YC-1 in differentiated PC12 cells.

Differentiated PC12 cells were incubated with YC-1 (10 µM) in the presence of Aβ25–35 (10 µM) for 24 h and ODQ (10 µM; a guanylyl cyclase inhibitor) was added to the medium 30 min before the addition of YC-1. The expression of (A) μ-calpain, (B) p25, and (C) p-tau was determined by Western blotting. All data are means ± SD of five independent observations with different cell passages and on different days. Depicted are a typical display of protein production (upper panel) and the statistical analysis of the changes of protein (lower panel). The typical data (β-actin) obtain from the same nitrocellulose membrane. *P#P25–35-treated cells.