1. Despite widespread recognition of its great promise to aid decision-making in environmental management, the applied use of metabarcoding requires improvements to reduce the multiple errors that
A custom mcrA gene (Methyl-coenzyme M reductase A) reference database with 39,544 sequences and assigned taxonomy for studying methanogens with metabarcoding. Sequences in database were extracted from
In this repo, the crest4 and the RDPClassifier oriented databases used in PEMA will be versioned. We list the provided databases per marker gene: Marker Gene crest4 Databases RDPClassifier Databas
Five emptyings of a Malaise trap (Western Germany) were processed with DNA metabarcoding. The study compares different methods of tissue homogenisation as well as the overlap between subsamples at ext