1SXTwt is strain RF146. 2Loss was calculated as indicated in materials and methods. 3Exconjugant formation was calculated as the number of exconjugant cfu/number of donor cfu. The data is from a repre
The goal of the study was to obtain plasmid containing the SAT1 Flipper knockout cassette along with upstream and downstream gene fragments (STR2) and to introduce a cleavage site for restriction enzy