Melting temperature (<i>T</i><sub>m</sub>) and cell viability of apoSOD1 protein variants.
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a.The midpoint of the thermal unfolding transition monitored by CD, as derived by fitting of a sigmoidal function (Eq. 3), using a ΔCp value of 1.4 kcal mol−1 K−1. This value was derived from the slope of ΔHU/F(Tm) vs. Tm for the first 20 mutants in Table 1 (data not shown). b.Mean cell viability expressed as percentage of buffer control with monomeric apoSOD1 [C6/111A;F50E;G51E] set to 0%. Replicate values are displayed in Figure 3A. c.Min/Max represents the minimum and maximum viability response observed for the individual mutations. d.Number of cell viability experiments for the individual SOD1 mutations.
创建时间：
2012-04-25
