Wetland Soil Characterization and Methane Production Impacted by Nickel Addition, Argonne and Tims Branch Wetlands, September and October 2020

Abstract: Freshwater wetland soils are foci of biogeochemical cycling as they serve as key sources of methane to the atmosphere. An array of metalloenzymes is essential to anaerobic microbial carbon transformations. Nickel is notably recognized as playing key roles in the enzymatic pathways of methanogenesis. Low availability of trace metals limits microbial element cycling in laboratory studies, but the occurrence of such limitations in natural subsurface aquatic systems is poorly understood. Microcosm incubation studies were carried out using two distinct wetland soils, one from a marsh wetland and the second from a riparian wetland, to explore the effect of dissolved Ni concentrations on methane production. Data are provided for wetland soil characterization and soil incubation experiments using materials from marsh wetlands at Argonne National Laboratory and riparian wetlands in the Tims Branch watershed at Savannah River National Laboratory. The characterization data consists soil carbon, nitrogen, sulfur, and iron contents plus as well as the solid-phase concentrations of copper, nickel, cobalt, and zinc, bioessential trace metals that may limits microbial metabolic process if they have low availability. The data for the soil incubation experiments include fluid pH, fluid dissolved trace metal concentrations, and cumulative methane production. Three soil incubations are reported: marsh wetland soil with increasing nickel addition, marsh wetland soil in sulfate-free water with increasing nickel addition, and riparian wetland soil with increasing nickel addition. All data are provided in text-based CSV format with header sections indicating the data contained in each file and the corresponding units. Note that "u" is used in place of Greek lower case mu to indicate the micro prefix on units. A Table of Contents file (Yan_Soil_Incubations_2020_TOC.txt) provides an index for the data contained in the individual files.摘要：淡水湿地土壤是生物地球化学循环的核心区域，同时也是大气甲烷的重要排放源。多种金属酶参与厌氧微生物的碳转化过程，其中镍在产甲烷的酶促通路中发挥的关键作用已得到学界广泛认可。实验室研究表明，微量金属有效供给不足会限制微生物的元素循环过程，但目前学界对自然地下水生系统中此类限制的发生情况仍知之甚少。本研究采用两种不同湿地土壤开展微宇宙培养实验，分别采集自沼泽湿地与河岸湿地，以探究溶解态镍浓度对甲烷产生过程的影响。本数据集涵盖土壤表征与土壤培养实验两类数据，其中土壤样品分别采集自美国阿贡国家实验室（Argonne National Laboratory）的沼泽湿地，以及美国萨凡纳河国家实验室（Savannah River National Laboratory）廷斯支流流域（Tims Branch watershed）的河岸湿地。土壤表征数据包括土壤碳、氮、硫、铁含量，以及铜、镍、钴、锌等生物必需微量金属的固相浓度——当这类金属有效供给不足时，可能会限制微生物的代谢过程。土壤培养实验数据则包含培养液pH值、溶解态微量金属浓度与累积甲烷生成量。本研究共设置三组土壤培养实验组：添加梯度浓度镍的沼泽湿地土壤组、无硫酸盐水环境下添加梯度浓度镍的沼泽湿地土壤组，以及添加梯度浓度镍的河岸湿地土壤组。所有数据均以文本型CSV格式存储，文件头部的表头字段标注了各文件包含的数据内容及对应单位。请注意：数据中使用字母‘u’替代希腊小写μ作为单位中的微（micro）前缀。目录文件Yan_Soil_Incubations_2020_TOC.txt可作为各单个数据文件的索引目录。