RNA extraction from wild-caught asian longhorned tick

Illumina HiseqX Ten system, 150PE sequencing(10Gb/sample)WorkflowRNA extraction -> Ribosomal RNA remove -> Dnase treatment -> cDNA synthesis -> Enzyme fragmentation -> Adapter ligation -> Size fraction -> Library QC -> Sequencing1-IN; RNA extraction from Haemaphysalis longicornis(whole body nymph, n=40) using Beniprep viral RNA extraction kit4-IA; RNA extraction from Haemaphysalis longicornis(whole body female adult, n=28) using Beniprep viral RNA extraction kit5-QN; RNA extraction from Haemaphysalis longicornis(whole body nymph, n=40) using Qiaamp viral RNA extraction kit6-QA; RNA extraction from Haemaphysalis longicornis(whole body female adult, n=28) using Qiaamp viral RNA extraction kit