Aerosol delivery of star polymer-siRNA nanoparticles as a therapeutic strategy to inhibit lung tumor growth

These datasets were used for the manuscript "Aerosol delivery of star polymer-siRNA nanoparticles as a therapeutic strategy to inhibit lung tumor growth". Here we performed an in-depth characterization of cell uptake, intracellular trafficking, and gene silencing activity of miktoarm star polymer (PDMAEMA-POEGMA) nanoparticles (star nanoparticles) complexed to siRNA in lung cancer cells. We also investigated the potential of nebulized star-siRNA nanoparticles to accumulate into orthotopic mouse lung tumors to inhibit the expression of two genes (βIII-tubulin, PLK1) which: 1) are highly expressed in lung cancer cells; 2) play an important role in promoting tumor growth; and 3) are difficult to inhibit using chemical drugs. All raw data are available below and quantitative analysis can be reproduced from this dataset.本数据集用于论文《气溶胶递送星形聚合物-siRNA纳米颗粒作为抑制肺肿瘤生长的治疗策略》。本研究对肺癌细胞中与小干扰RNA（siRNA）复合的杂臂星形聚合物（miktoarm star polymer，PDMAEMA-POEGMA）纳米颗粒（星形纳米颗粒）的细胞摄取、细胞内转运及基因沉默活性进行了深入表征。同时，本研究探究了雾化星形-siRNA纳米颗粒在原位小鼠肺肿瘤中富集的潜力，以抑制两种基因的表达：βIII-微管蛋白（βIII-tubulin）与PLK1，这两种基因具备以下特征：1）在肺癌细胞中高表达；2）在促进肿瘤生长过程中发挥重要作用；3）难以通过化学药物实现抑制。所有原始数据均已公开于此，且可通过本数据集复现定量分析结果。