Bioenergetic control of synaptic plasticity by astrocytes during acute stress

Aldh1l1CreERT2-RiboTag mice were used to purify and sequence astrocyte actively translated mRNAs from acute stress mouse models (20 minute forced swim) and non stressed controls. Overall design: Four weeks after tamoxifen injection, somatosensory cortex was homogenized and RNA was purified from (i) cleared lysate as the input and control, and (ii) astrocyte-specific ribosome-associated RNA precipitated via a hemagglutinin (HA) tag. Stress mouse astrocyte transcriptomes were compared to non stressed.