Context dependent function of transcriptional regulator Rap1 in gene silencing and activation [ChIPseq I]

As part of a study on the role of Rap1 at the promoters of HML and MAT in S cerevisiae, we endogenously tagged Rap1 with a 3xV5 tag and performed ChIPseq in a variety of genetic backgrounds including: silenced (SIR); unsilenced (sir1∆/sir3∆/sir4∆); in strains witha wild type alpha2 promoter (wtpromoter); or with a point mutation at the promoter (mutpromoter).本研究旨在探究酿酒酵母（S. cerevisiae）中Rap1在HML与MAT基因启动子处的调控作用，我们通过内源性标记方式为Rap1融合了3xV5标签，并在多种遗传背景下开展了染色质免疫共沉淀测序（ChIP-seq）实验，所涉及的遗传背景包括：沉默型（SIR）菌株、去沉默型（sir1Δ/sir3Δ/sir4Δ）菌株、携带野生型α2启动子（wtpromoter）的菌株，以及携带启动子点突变（mutpromoter）的菌株。