Androgen Receptor Signaling Regulates the Transcriptome of Prostate Cancer Cells by Modulating Global Alternative Splicing. Androgen Receptor Signaling Regulates the Transcriptome of Prostate Cancer Cells by Modulating Global Alternative Splicing

HTA2.0 affy array was used to study the effect of androgen inhibitors on alternative splicing in LNCaP cells Overall design: To study the effect of pharmacological manipulation of AR signaling in prostate cancer cells, we performed expression profiling of LNCaP cells that were cultured for 72 hours in charcoal-stripped fetal bovine serum and stimulated with 10nM AR agonist dihydrotestosterone (DHT) or 10uM of the AR antagonist casodex for 24 hours. 3 biological replicates for each group.