NGS analysis of HTT editing in vitro and in vivo

Tables S2-S3: Next-generation sequencing (NGS; MiSeq) analysis of on-target editing evaluating deletions and insertions (indels) in the CRISPR-HTT group in vitro and in vivo. For each experiment (in vitro and in vivo), the dataset is composed of an excel file giving for each read, the length, the alignment score (to the reference sequence), the CIGAR string and whether indels were detected closed to expected cleavage site or not. Tables S4-S5: Potential off-target sites for sgHTT and sgCas9 were assessed with the Bioconductor package CRISPRseek with default parameters. The tables report the potential off-targets for each sgRNA with a score equal or above 1.附表S2至S3：针对CRISPR-HTT组的体外及体内样本，采用下一代测序（NGS；MiSeq）分析其靶向编辑所产生的插入缺失（indels）。针对每项体外与体内实验，本数据集包含一份Excel文件，其中记录了每条测序读段的长度、与参考序列的比对评分、CIGAR字符串，以及是否在预期切割位点附近检测到插入缺失。 附表S4至S5：采用Bioconductor软件包CRISPRseek的默认参数，对sgHTT与sgCas9的潜在脱靶位点进行评估。附表中记录了每条单向导RNA（sgRNA, single-guide RNA）评分不低于1的潜在脱靶位点。