Architecture mechanics mediated osteogenic progression in bone regeneration of artificial scaffolds

Scaffold architecture exerts a considerable influence on the osteogenic effect through stress transmission, as the deformation of scaffolds alters the mechanical microenvironment of cells adhering to scaffold surface. Despite extensive researches on bone regeneration influenced by scaffold architecture, present studies have not addressed the biological mechanism underlying scaffold architecture-induced stress stimulation (SASS) on cells yet, posing a great challenge in revealing the biomechanical cues between scaffold architecture and osteogenic progression. Therefore, Graphite (GP), Fullerene (FL), and Diamond (DM) scaffolds with gradient stress-stimulation to cells after deformation were prepared. Moreover, we analyzed the results of the finite element simulation of the compression and recovery process of the three types of scaffolds, and tested the macroscopic mechanical and viscoelastic properties. The cellular biomechanical mechanisms of SASS through single-cell RNA sequencing indi..., , # Architecture mechanics mediated osteogenic progression in bone regeneration of artificial scaffolds Dataset DOI: [10.5061/dryad.dr7sqvb8w](10.5061/dryad.dr7sqvb8w) ## Description of the data and file structure We summarized the finite element simulation of the micro-strain distribution, macro-mechanical, and viscoelastic properties of GP, FL, and DM scaffolds during the compression and recovery process. One month after implantation in the femur of rats, the new bone at the defect was removed, and single-cell sequencing was performed. The KEGG, GO enrichment, and gene expression differences of mesenchymal stem cells (MSCs), osteoclasts (OCs), and osteoblasts (OBs) cell clusters in the three scaffolds were analyzed. In the *in vivo* experiments, the parameters of new bone, semi-quantitative analysis of IHC and IF were analyzed. ### Files and variables #### File: Data\_files.zip **Description:**Â **Porosity&surface-volume ratio.xlsx**: Porosity and surface-volume ratio of GP, FL, ...,支架结构通过应力传递对成骨效应产生显著影响，支架形变会改变黏附于其表面的细胞所处的力学微环境。尽管已有大量关于支架结构影响骨再生的研究，但目前尚未阐明支架结构诱导的细胞应力刺激（scaffold architecture-induced stress stimulation, SASS）背后的生物学机制，这为揭示支架结构与成骨进程之间的生物力学信号带来了巨大挑战。为此，本研究制备了形变后对细胞具有梯度应力刺激的石墨（Graphite, GP）、富勒烯（Fullerene, FL）与金刚石（Diamond, DM）支架。此外，我们分析了三种支架压缩与恢复过程的有限元模拟结果，并测试了其宏观力学与黏弹性性能。本研究通过单细胞RNA测序探究了SASS的细胞生物力学机制，相关内容详见原文截断部分。 # 人工支架骨再生中力学架构介导的成骨进程 数据集DOI：[10.5061/dryad.dr7sqvb8w](10.5061/dryad.dr7sqvb8w) ## 数据集与文件结构说明 本研究汇总了GP、FL、DM三种支架在压缩与恢复过程中的微应变分布、宏观力学与黏弹性性能的有限元模拟数据。将支架植入大鼠股骨缺损部位1个月后，收集缺损处新生骨组织并开展单细胞测序。本研究分析了三种支架中间充质干细胞（mesenchymal stem cells, MSCs）、破骨细胞（osteoclasts, OCs）与成骨细胞（osteoblasts, OBs）细胞簇的KEGG富集分析、GO富集分析结果以及基因表达差异。在体内（in vivo）实验中，本研究还分析了新生骨相关参数以及免疫组化（IHC）与免疫荧光（IF）的半定量分析结果。 ### 文件与变量说明 #### 压缩包：Data_files.zip **描述：** **Porosity&surface-volume ratio.xlsx**：包含GP、FL等支架的孔隙率与比表面积数据