Primer melting temperature is given under (as calculated by the wEMBOSS [57] program ‘dan’). ‘Length’ denotes the length of the amplicon in basepairs, ‘Reference’ indicates from which publication the
aAll sequences are based on the sequence of the J35 isolate (GenBank accession no. AY386330).bGGATCC is a restriction site of BamHI, AAGCTT is a restriction site of HindIII.
