Water Quality Data (Extensive) from the Taylor Slough, Everglades National Park (FCE), South Florida from July 1999 to Present

Water quality samples are being collected using ISCO autosamplers at all wetland sites (that is, all sites except TS/Ph-9, 10, and 11). The autosamplers contain 24 1L bottles. Water is sampled by programming the autosamplers to take composite samples once every 3 days. These samples are a composite of four 250mL subsamples drawn every 18 hours (a sampling scheme that captures a dawn, noon, dusk, and midnight sample in every three day composite). The samples are collected every 3-4 weeks and analyzed for total phosphorus (TP), total nitrogen (TN), and salinity. When sites are visited to collect these samples, we also collect a grab sample that is immediately put on ice. A portion of these grab samples is filtered through a Whatman GF/F filter immediately upon return to the lab, and the filtered samples are analyzed for inorganic nutrients such as NO2-, NO3-, NH4+, SRP, and DOC. The unfiltered fraction of these grab samples is analyzed for TP, TN, and TOC. We use these monthly grab samples to generate relationships between TP and SRP, and between TN and NO2- + NO3- + NH4+. Dissolved nutrients are measured using standard rapid flow analyzer (RFA) techniques. TP is analyzed with a modified Solorzano and Sharp (1980) technique. TN is measured with an Antec TN analyzer, TOC and DOC are quantified on a Shimadzu TOC Analyzer, and salinity is measured with an YSI conductivity meter. In addition to the regular water quality monitoring, we use the rain level actuators at all freshwater sites to trigger water sampling after rain events exceed a given threshold of duration and/or intensity. As currently programmed, when the threshold of = 2.5 cm of rain per hour is passed, the autosampler at that site collects a 500mL sample 30 minutes after the threshold has been reached. Rain event samples are collected, treated, and analyzed as all other water quality samples.研究团队将使用ISCO自动采样器（ISCO autosamplers）在所有湿地样点开展水质样本采集工作（TS/Ph-9、10、11号样点除外）。该自动采样器配备24个1L容积的采样瓶。通过编程设置，自动采样器每3天采集一次混合水样：每18小时采集一份250mL子样本，每3天的混合样本由4份子样本混合而成，该采样方案可在每3天的混合样本中覆盖黎明、正午、黄昏与午夜四个时段的水样。样本采集周期为每3至4周一次，检测指标包括总磷（TP）、总氮（TN）与盐度。在前往样点采集上述水样的同时，研究团队还会采集瞬时水样（grab sample），并即刻将其置于冰上保存。返回实验室后，即刻使用Whatman GF/F滤膜（Whatman GF/F filter）对部分瞬时水样进行过滤：过滤后的水样用于检测亚硝酸盐（NO₂⁻）、硝酸盐（NO₃⁻）、氨氮（NH₄⁺）、可溶性活性磷（SRP）以及溶解性有机碳（DOC）等无机营养盐；未经过滤的水样则用于检测总磷、总氮与总有机碳（TOC）。研究团队利用月度采集的瞬时水样，建立总磷与可溶性活性磷、总氮与亚硝酸盐+硝酸盐+氨氮之间的相关关系。溶解态营养盐采用标准快速流动分析仪（rapid flow analyzer, RFA）技术进行检测；总磷采用改良后的Solorzano与Sharp（1980）方法检测；总氮使用Antec总氮分析仪完成测定；总有机碳与溶解性有机碳通过岛津（Shimadzu）TOC分析仪定量；盐度则采用YSI电导仪（YSI conductivity meter）进行测量。除常规水质监测外，研究团队还在所有淡水样点部署雨量触发装置，当降雨事件的时长和/或强度达到设定阈值时启动水样采集。当前的编程设置为：当每小时降雨量达到2.5 cm及以上时，对应样点的自动采样器将在阈值达标后30分钟采集500mL水样。降雨事件触发的水样与其他常规水质样本采用相同的处理与分析流程。