The Hubbard Brook Stream Ecology Record: Algal Biomass (Chlorophyll-a), 2018 - ongoing

The Hubbard Brook Stream Ecology record is a companion dataset to the Hubbard Brook Watershed Stream and Precipitation Chemistry record. The Stream Ecology record started in 2018 and HBWatER collects ecological samples from seven gauged watersheds: Watersheds 1 through 6 and Watershed 9. HBWatER measures algal biomass, aquatic invertebrate emergence, and stream decomposition by measuring (1) chlorophyll-a on tiles and artificial moss, which approximate algal biomass growth on bare rock and bryophyte mats, (2) preserved algal biomass on artificial moss substrates in Lugol’s Iodine solution, (3) aquatic invertebrate emergence on replicate sticky traps placed above the stream, and (4) stream decomposition through leaf litter pack and cotton strip decay. To complement these ecological records, HBWaTER installed light sensors and field cameras to obtain better information about the light and stream environment daily. Three replicate light sensors that take sub-daily measurements of light level intensity are placed at each watershed at the weir pond (full-sun), and two under the canopy (partial shade). Field cameras take daily photos at noon of the stream canopy and the stream channel. While many studies at Hubbard Brook have measured algal biomass, aquatic invertebrates, and stream decomposition, they are scattered in locations across the valley, were performed at non-continuous times, and use various semi-comparable methods. The HBWatER Stream Ecology record was created to address this gap and systematically measure any long-term changes in the organisms living in the stream. The collection of HBWatER samples is currently sustained by Tammy Wooster (Cary IES) and analyses of these samples has been performed by Heather Malcom (Cary IES), Audrey Thellman (Duke), Steve Anderson (Duke), Geoff Wilson (Cary IES), and Adam Rok (Duke). The dataset is curated and maintained by a team of researchers: Chris Solomon (Cary IES), Emma Rosi (Cary IES), and Emily Bernhardt (Duke). Current Financial Support for HBWatER is provided by NSF LTREB #1907683 and the NSF LTER #XXX (I think you mentioned that you know the LTER fund code for this). Data were gathered as part of the Hubbard Brook Ecosystem Study (HBES). The HBES is a collaborative effort at the Hubbard Brook Experimental Forest, which is operated and maintained by the USDA Forest Service, Northern Research Station.《哈伯德布鲁克溪流生态数据集》（Hubbard Brook Stream Ecology Record）是《哈伯德布鲁克流域溪流与降水化学数据集》（Hubbard Brook Watershed Stream and Precipitation Chemistry Record）的配套数据集。该数据集始建于2018年，HBWatER项目从7个已布设测流堰的流域采集生态样本：1至6号流域与9号流域。 HBWatER的测定指标涵盖藻类生物量、水生无脊椎动物羽化量与溪流分解速率，具体测定方式包括：(1) 检测基质片与人工苔藓上的叶绿素-a（chlorophyll-a），以此模拟裸岩与苔藓垫上的藻类生物量生长状况；(2) 对卢戈氏碘液（Lugol’s Iodine solution）中保存的人工苔藓基质上的藻类生物量进行固定检测；(3) 在溪流上方布设重复式粘捕陷阱，采集水生无脊椎动物的羽化个体；(4) 通过落叶袋（leaf litter pack）与棉条分解实验测定溪流分解速率。 为补充上述生态观测数据，HBWatER还布设了光传感器与野外相机，以每日获取更详实的光照与溪流环境信息。每个流域的堰塘（全日照环境）处布设3台可获取亚日级光照强度数据的重复式光传感器，另有2台布设于林冠下（半遮阴环境）。野外相机于每日正午拍摄溪流林冠与河道的影像。 尽管哈伯德布鲁克（Hubbard Brook）的诸多研究已对藻类生物量、水生无脊椎动物与溪流分解速率进行过测定，但这些研究的采样点分散于山谷各处、观测时间不连续，且采用了多种半可比的实验方法。HBWatER溪流生态数据集正是为填补这一研究空白而创建，旨在系统监测溪流生境中生物群落的长期变化。 目前，HBWatER样本的采集工作由塔米·伍斯特（Tammy Wooster，卡里环境研究所（Cary Institute of Ecosystem Studies, CARY IES））负责，样本分析工作则由希瑟·马尔科姆（Heather Malcom，CARY IES）、奥黛丽·特尔曼（Audrey Thellman，杜克大学（Duke University, Duke））、史蒂夫·安德森（Steve Anderson，Duke）、杰夫·威尔逊（Geoff Wilson，CARY IES）与亚当·罗克（Adam Rok，Duke）共同完成。该数据集由克里斯·所罗门（Chris Solomon，CARY IES）、艾玛·罗西（Emma Rosi，CARY IES）与埃米莉·伯恩哈特（Emily Bernhardt，Duke）组成的研究团队进行管理与维护。 HBWatER当前的经费支持来自美国国家科学基金会（National Science Foundation, NSF）长期生态生物学研究拨款项目（LTREB #1907683）以及NSF长期生态研究网络（LTER）拨款项目#XXX（据此前沟通可知该项目的LTER资助编号）。本数据集的数据采集工作作为哈伯德布鲁克生态系统研究（Hubbard Brook Ecosystem Study, HBES）的一部分开展。HBES是依托哈伯德布鲁克实验林（Hubbard Brook Experimental Forest）开展的协作研究项目，该实验林由美国农业部林务局（USDA Forest Service）北方研究站（Northern Research Station）运营维护。