Squash preparations and living cells showing chromosome arrangement in mitosis, meiosis I, and meiosis II in green lacewing (Chrysoperla rufilabris) males

In previously-studied species of insects in the order Neuroptera, sex chromosomes in males exhibit a phenomenon called \"distance segregation,\" where the X and Y chromosomes are separated and associated with opposite poles from prometaphase I-anaphase I. This study aimed to determine the chromosome number and sex determination of a previously unstudied Neuropteran insect, the green lacewing Chrysoperla rufilabris (Burmeister, 1839).  Following determination of chromosome number and sex determination we examined images of fixed and living specimens to determine if distance segregation was observed. This dataset includes all of the raw images of living and aceto-orcein squashed cells that were observed to determine chromosome number, sex determination, and existence of distance segregation., Testes of Chrysoperla rufilabris were removed from adult male abdomens.   For aceto-orcein squashes, isolated testes were fixed in 3:1 ethanol/acetic acid for 5 minutes, rinsed 30 seconds in distilled water, and stained in filtered aceto-orcein (2.5 g orcein in 100 mL 45% (v/v) acetic acid) for 3 minutes.  Stained testes were placed on a slide in a drop of 45% acetic acid.  A coverslip was placed over the testes and uniform pressure was applied by hand on the coverslip.  For living-cell preparations, testes were placed under a thin layer of Kel-F #10 oil (Ohio Valley Specialty Company, Marietta, Ohio) in a culture chamber on a coverslip.  Testes contents were spread under the oil and living cells were immediately imaged. Imaging was done on a Nikon Eclipse TS100 microscope (Nikon Instruments, Tokyo, Japan) equipped with a 100X, 1.25 NA phase-contrast, oil immersion objective.  Images were collected either with a View 4K HD camera (Microscope Central, F..., # Squash preparations and living cells showing chromosome arrangement in mitosis, meiosis I, and meiosis II in green lacewing (*Chrysoperla rufilabris*) males Dataset DOI: [10.5061/dryad.15dv41p8f](10.5061/dryad.15dv41p8f) ## Description of the data and file structure This dataset contains the raw image files required to study chromosome number, alignment, and position during mitosis, meiosis I, and meiosis II in adult males of the green lacewing *Chrysoperla rufilabris* (Burmeister, 1839). Images of aceto-orcein squashes of testes and living spermatocytes are included in this dataset. Preparations were imaged using a Nikon Eclipse TS100 microscope equipped with a 100X, 1.25 NA phase-contrast, oil immersion objective. Images of aceto-orcein squashes were collected using a View 4K HD camera (Microscope Central, Feasterville, PA, USA) using InFocus software version x64 (Microscope Central). Images of living cells were either collected using a View 4K HD camera using InFocus softwa...,针对此前已被研究的脉翅目（Neuroptera）昆虫类群，雄性个体的性染色体存在一种名为“距离分离（distance segregation）”的现象：即X与Y染色体在减数分裂前期I到后期I（prometaphase I-anaphase I）阶段发生分离，并分别趋向两极。本研究旨在明确一种此前未被研究的脉翅目昆虫——黄足草蛉（Chrysoperla rufilabris，Burmeister, 1839）的染色体数目与性别决定方式。在确定染色体数目与性别决定方式后，我们通过观察固定标本与活体细胞标本的成像结果，以验证是否存在距离分离现象。本数据集包含所有用于分析染色体数目、性别决定方式及距离分离现象是否存在的活体细胞与醋酸地衣红（aceto-orcein）压片细胞的原始成像文件。实验材料取自成年雄性黄足草蛉腹部的精巢。 醋酸地衣红压片制备流程如下：将分离得到的精巢置于3:1的乙醇/冰乙酸固定液中固定5分钟，随后用蒸馏水漂洗30秒，再于过滤后的醋酸地衣红染液（2.5g地衣红溶于100mL 45%（体积分数）冰乙酸）中染色3分钟。将染色后的精巢置于载玻片上，滴加一滴45%冰乙酸，盖上盖玻片后手动施加均匀压力使组织铺展。 活体细胞标本制备流程如下：将精巢置于盖玻片上的培养腔中，覆盖一层薄的Kel-F #10油（俄亥俄谷特种公司，玛丽埃塔，俄亥俄州），使精巢内容物在油层下铺展后，立即对活体细胞进行成像。 成像实验使用搭载100×、1.25NA相差（phase-contrast）油浸物镜的Nikon Eclipse TS100显微镜（尼康仪器公司，东京，日本）完成。图像采集或使用View 4K HD相机（Microscope Central，……）。本数据集涵盖展示黄足草蛉（Chrysoperla rufilabris）雄性个体有丝分裂、减数分裂I及减数分裂II过程中染色体排布的压片标本与活体细胞成像结果。 数据集DOI：10.5061/dryad.15dv41p8f ### 数据与文件结构说明 本数据集包含用于研究黄足草蛉（Chrysoperla rufilabris，Burmeister, 1839）成年雄性个体有丝分裂、减数分裂I及减数分裂II过程中染色体数目、排列方式与位置的原始成像文件。数据集涵盖精巢醋酸地衣红压片标本与活体细胞精母细胞的成像结果。所有标本均使用搭载100×、1.25NA相差油浸物镜的Nikon Eclipse TS100显微镜（尼康仪器公司，东京，日本）进行成像。其中醋酸地衣红压片标本的图像通过View 4K HD相机（Microscope Central，费斯特维尔，宾夕法尼亚州，美国）结合InFocus x64软件（Microscope Central）采集。活体细胞图像则通过View 4K HD相机结合InFocus软件采集……