Single particle cryo-EM structure of homohexameric 2-oxoglutarate dehydrogenase OdhA from Corynebacterium glutamicum following reaction with the 2-oxoglutarate analogue succinyl phosphonate

Single particle cryo-EM structure of homohexameric 2-oxoglutarate dehydrogenase OdhA from Corynebacterium glutamicum following reaction with the 2-oxoglutarate analogue succinyl phosphonate Descriptor: (4~{S})-4-[(2~{R})-3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-4-methyl-5-[2-[oxidanyl(phosphonooxy)phosphoryl]oxyethyl]-2~{H}-1,3-thiazol-2-yl]-4-oxidanyl-4-phosphono-butanoic acid, 2-oxoglutarate dehydrogenase E1/E2 component, ACETYL COENZYME *A, ... Authors: Yang, L, Mechaly, A.M, Bellinzoni, M. Deposit date: 2023-05-24 Release date: 2023-08-16 Last modified: 2023-08-23 Method: ELECTRON MICROSCOPY (2.26 Å) Cite: High resolution cryo-EM and crystallographic snapshots of the actinobacterial two-in-one 2-oxoglutarate dehydrogenase. Nat Commun, 14, 2023谷氨酸棒杆菌（Corynebacterium glutamicum）来源的同源六聚体2-氧戊二酸脱氢酶OdhA，与2-氧戊二酸类似物琥珀酰膦酸酯反应后的单颗粒冷冻电镜（cryo-EM）结构。 描述信息：配体为(4S)-4-[(2R)-3-[(4-氨基-2-甲基-嘧啶-5-基)甲基]-4-甲基-5-[2-[羟基(膦酰氧基)膦酰基]氧基乙基]-2H-1,3-噻唑-2-基]-4-羟基-4-膦酰基-丁酸，该酶属于2-氧戊二酸脱氢酶E1/E2组分，涉及乙酰辅酶A（ACETYL COENZYME *A）…… 作者：Yang, L、Mechaly, A.M、Bellinzoni, M。 沉积日期：2023-05-24；发布日期：2023-08-16；最后修改日期：2023-08-23。 方法：电子显微镜（2.26 Å）。 引用文献：放线菌“二合一”2-氧戊二酸脱氢酶的高分辨率冷冻电镜与晶体快照，《自然-通讯》（Nat Commun），14卷，2023年。