CRISPR Design Tool and Protocol

Figshare2016-01-19 更新2026-04-08 收录

CRISPRi基因编辑

sgRNA设计

数据链接：

https://figshare.com/articles/dataset/CRISPR_Design_Tool/1117899/15 数据链接链接失效反馈

官方服务：

资源简介：

Excel tool with documentation for designing sgRNAs from the Casellas Lab, NIH. Updated tool and documentation on 11/29/5. Enjoy!

创建时间：

2015-11-30

Use of CRISPR/Cas9 with homology-directed repair to silence the human Topoisomerase IIα intron-19 5' splice site: Generation of etoposide resistance in human leukemia K562 cells. Use of CRISPR/Cas9 with homology-directed repair to silence the human Topoisomerase IIα intron-19 5' splice site: Generation of etoposide resistance in human leukemia K562 cells

CRISPRi基因编辑

治疗抗性

Results support the important role of both TOP2α/170 and TOP2α/90 as determinants of sensitivity/resistance to TOP2α-targeting agents. Overall design: CRISPR/Cas9 with homology-directed repair was uti

NIAID Data Ecosystem40

sgRNA sequences used for CRISPR targeting of TET1.

CRISPRi基因编辑

sgRNA序列设计

sgRNA sequences used for CRISPR targeting of TET1.

Figshare2019-06-19 更新20

EGAS00001006170-sc-2023-10-20T08:25:08Z - samples

DNA提取

CRISPRi基因编辑

8 cell pellet samples for genomic DNA extraction. CRISPR PCR1 and PCR2 indexing - Please use standard Kosuke primers.EGA dataset EGAD00001012233

NIAID Data Ecosystem00

File S2.zip

CRISPRi基因编辑

基因敲入序列分析

Annotated sequence files in .ape format (comparable with Genbank) of knock-ins generated by CRISPR/Cas9-mediated genome editing. Unmodified genomic sequence is also provided.

Figshare2023-03-14 更新20

CRISPR RNA-seq from HepG2 (ENCSR624YDI)

CRISPRi基因编辑

转录组分析

RNA-seq on HepG2 cells treated with a CRISPR gRNA against MAK16. (MAK16-BGHcLV12) For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/R

NIAID Data Ecosystem10

CRISPR Design Tool and Protocol

资源简介：

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