Phage selection drives resistance-virulence trade-offs in Ralstonia solanacearum plant pathogenic bacterium irrespective of the growth temperature

While temperature has been shown to affect the survival and growth of bacteria and their phage parasites, it is unclear if trade-offs between phage resistance and other bacterial traits depend on the temperature. Here, we experimentally compared the evolution of phage resistance-virulence trade-offs and underlying molecular mechanisms in phytopathogenic Ralstonia solanacearum bacterium at 25 °C and 35 °C temperature environments. We found that experimental growth conditions selected for small colony variants (SCVs) with increased growth rate and mutations in the quorum-sensing (QS) signalling receptor gene, phcS. Interestingly, SCVs were also phage-resistant and reached higher frequencies in the presence of phages in both temperature environments. Evolving phage resistance was costly in terms of reduced carrying capacity, biofilm formation and reduced virulence in planta possibly due to loss of QS-mediated expression of key virulence genes. We also observed mucoid phage-resistant coloni..., , , # Phage selection drives resistance-virulence trade-offs in Ralstonia solanacearum plant pathogenic bacterium irrespective of the growth temperature [https://doi.org/10.5061/dryad.tdz08kq5d](https://doi.org/10.5061/dryad.tdz08kq5d) This is an experiment focused on the coevolution of Ralstonia solanacearum and phages. We gathered information concerning the growth of R. solanacearum and the presence of phages at various stages of the experiment. Subsequent to isolating the evolved strains, we conducted assessments of their fundamental characteristics, encompassing growth parameters, motility, biofilm formation capability, and pathogenicity. Furthermore, we performed genomic analysis on these evolved strains, identified pivotal genes through SNP analysis, and corroborated the functionality of these genes via experimental assays. ## Description of the data and file structure Datasets included: 1. transfer OD • every two days’ OD600 value of each replicate in R. solanacearum -phage s...已有研究证实温度可影响细菌及其噬菌体寄生物的存活与生长，但目前尚不明确噬菌体抗性与其他细菌性状间的权衡关系是否依赖于温度。本研究分别在25℃与35℃的温度环境下，通过实验比较了植物致病青枯雷尔氏菌（Ralstonia solanacearum）的噬菌体抗性-毒力权衡关系及其潜在分子机制。 研究发现，实验生长条件筛选出了生长速率提升且群体感应（quorum-sensing, QS）信号受体基因phcS发生突变的小菌落变异株（small colony variants, SCVs）。有趣的是，小菌落变异株同时具备噬菌体抗性，且在两种温度环境下与噬菌体共存时均能达到更高的种群频率。获得噬菌体抗性会带来适应性代价，具体表现为植株内承载能力下降、生物膜形成能力减弱以及毒力降低，这可能源于群体感应介导的关键毒力基因表达缺失。本研究还观察到了黏液状噬菌体抗性菌落…… # 噬菌体选择驱动植物致病型青枯雷尔氏菌的抗性-毒力权衡，且不受生长温度影响 [https://doi.org/10.5061/dryad.tdz08kq5d](https://doi.org/10.5061/dryad.tdz08kq5d) 本实验聚焦于青枯雷尔氏菌与噬菌体的协同进化。研究收集了实验各阶段中青枯雷尔氏菌的生长状态及噬菌体丰度相关数据。在分离得到进化菌株后，我们对其基础生物学性状开展了评估，涵盖生长参数、运动能力、生物膜形成能力与致病性。此外，我们对这些进化菌株进行了基因组分析，通过单核苷酸多态性（single nucleotide polymorphism, SNP）分析鉴定出关键基因，并通过实验验证了这些基因的功能。 ## 数据与文件结构说明 本数据集包含： 1. 转接光密度值（transfer OD） • 青枯雷尔氏菌-噬菌体共培养体系中每个重复样本每两天的OD600值……