BRD3 Proteomics

BRD3-interacting proteins were isolated by immunoprecipitation using a BRD3-specific antibody from LPS-treated THP1-M155 and RAW264.7 cells. Equal volumes of immunoprecipitates from each sample were resolved on 4–12% Criterion XT Bis-Tris gels (Bio-Rad, Cat. #3450124), followed by mass spectrometry analysis.采用BRD3特异性抗体（BRD3-specific antibody），对经脂多糖（LPS）处理的THP1-M155及RAW264.7细胞进行免疫沉淀，从而分离得到BRD3相互作用蛋白（BRD3-interacting proteins）；将每份样本的免疫沉淀物等体积上样至4–12% Criterion XT Bis-Tris凝胶（伯乐Bio-Rad，货号Cat. #3450124）中进行电泳分离，随后开展质谱分析。