Data for: Functional unification of sex pheromone-receptive glomeruli in the invasive Turkestan cockroach derived from the genus Periplaneta

Supplementary movie file legends Supplementary movie 1 The right antennal lobe glomeruli of the male P. americana, showing two macroglomeruli responsive to periplanone-A (A-glomerulus) and periplanone-B (B-glomerulus). Supplementary movie 2 The right antennal lobe glomeruli of the male B. lateralis, showing a single macroglomerulus responsive to periplanone-A and periplanone-B (see text). Note that the glomerulus homologous to the A-glomerulus in P. americana has a size similar to those of ordinary glomeruli. Supplementary movie 3 Mating behavior of P. americana males evoked by the presentation of periplanone. Introducing periplanone-B (5 ng) soaked in filter paper evoked nearly instant mating behavior in males such as wing raising behavior oriented toward the pheromone source. Homsexual behavior with attempts to copulate with neighboring males was also observed. Supplementary movie 4 Behavior of B. lateralis males in response to the presentation of periplanone-B (5 ng) or natural sex pheromone extract (2 female unit). The males showed attraction to the pheromone source and carefully inspected it but never exhibited mating behavior, although the projection neuron that mediates this behavior is exquisitely sensitive to periplanone-B. The presentation of periplanone-A also never evoked mating behavior in the males. Supplementary movie 5 Mating behavior of B. lateralis males in response to the presentation of a female gently anesthetized with CO2. Immediately after careful inspection of the female with antennation, the males exhibited wing raising behavior and attempted to mate with the conspecific female.补充视频文件说明： 补充视频1 雄性美洲大蠊（P. americana）的右侧触角叶嗅小球，可见两个巨嗅小球，分别响应佩里普兰酮-A（periplanone-A，即A型嗅小球）与佩里普兰酮-B（periplanone-B）。 补充视频2 雄性B. lateralis的右侧触角叶嗅小球，可见单个对佩里普兰酮-A与佩里普兰酮-B均产生响应的巨嗅小球（详见正文）。需注意，该嗅小球与美洲大蠊A型嗅小球为同源结构，其尺寸与普通嗅小球相仿。 补充视频3 美洲大蠊雄性在佩里普兰酮诱导下的交配行为。将浸有5 ng佩里普兰酮-B（periplanone-B）的滤纸置于环境中，可快速诱导雄性展现朝向信息素源的振翅行为等交配动作，同时还观察到其试图与邻近雄性交配的同性行为。 补充视频4 B. lateralis雄性在施加佩里普兰酮-B（5 ng）或天然性信息素提取物（2雌性单位）时的行为表现。该雄性个体虽会向信息素源靠近并进行细致探查，但未展现出交配行为——尽管介导该行为的投射神经元对佩里普兰酮-B具有极高的敏感性。施加佩里普兰酮-A（periplanone-A）同样无法诱导该雄性产生交配行为。 补充视频5 B. lateralis雄性在接触经CO₂轻度麻醉的雌性个体时的行为表现。雄性通过触角触碰对雌性进行细致探查后，即刻展现振翅行为，并尝试与同种雌性完成交配。