Endogenous expression of inactive lysine deacetylases KDAC6 (HDAC6) and KDAC8 (HDAC8)

To investigate the roles of KDAC6 and KDAC8, we generated cell lines containing point mutations in HT1080 cells that result in endogenous expression of a catalytically-inactive variant. Expression profiling analysis was then performed on the resulting cell lines vs. wild HT1080 cells and cells treated with KDAC inhibitors tubastatin A or PCI-34051. Comparative gene expression profiling analysis of RNA-seq data for HT1080 cells, variants containing inactivated KDACs, and inhibitor treatments with 3-4 replicates