Therapeutic effects of EGCG in experimentally induced ulcerative colitis in rats via affecting inflammation and apoptosis

Ulcerative colitis (UC) is a chronic inflammatory bowel disease that affects the colon. Epigallocatechin gallate (EGCG), a compound found in green tea with antioxidant and anti-inflammatory properties. We aimed to investigate the impact of EGCG on inflammation and apoptotic pathways in UC rats. The study involved inducing UC in rats by administering acetic acid. The UC rats were then treated with EGCG. Colon samples were collected, measured then used to evaluate gene and protein expression of various factors, including NF-κB, TNF-α, SphK, MIP-1α, BCL2, and BAX, as well as the activities of caspase-3/8/9., Animals and treatment outlines In this study, 36 rats belonging to the Sprague-Dawley species and weighing between 180 and 200 grams were employed. The rats were divided into three groups, each consisting of twelve rats. Control group: Rats were subjected to mild anesthesia using ether followed by the administration of 2 ml of saline solution into the colon using a soft pediatric lubricated catheter. The rats were positioned horizontally for two minutes to prevent the saline from draining. Subsequently, they received a daily oral dose of 0.5% (w/v) methylcellulose and 2% (v/v) Tween 80, both dissolved in deionized water, for two weeks. Ucerative Colitis group: The rats were anesthetized with ether and subsequently administered 2 ml of 4% acetic acid via a gentle pediatric catheter into their colon. Following the administration, the rats were positioned horizontally for two minutes to promote the retention of the acetic acid. Ulcerative Colitis group treated with genistein: UC was induce..., , # Therapeutic effects of EGCG in experimentally induced ulcerative colitis in rats via affecting inflammation and apoptosis [https://doi.org/10.5061/dryad.n02v6wx6v](https://doi.org/10.5061/dryad.n02v6wx6v) ## Description of the data and file structure The uploaded data represented the experiment's results on colons separated from different rat groups used. The data are described in the following table: | Variable | Unit | Description | | :---------------------- | :------------------ | :-------------------------------------------------------------------------------------------------------------------------------------------------------------------------- | | Group |  | The name of the group. The study consists of three groups: the control group, the ulcerativ...溃疡性结肠炎（Ulcerative colitis, UC）是一种累及结肠的慢性炎症性肠病。表没食子儿茶素没食子酸酯（Epigallocatechin gallate, EGCG）是绿茶中提取的天然化合物，兼具抗氧化与抗炎活性。本研究旨在探究EGCG对溃疡性结肠炎大鼠炎症通路与凋亡通路的调控作用。实验通过乙酸灌肠诱导构建大鼠溃疡性结肠炎模型，随后给予EGCG干预。收集各组大鼠结肠组织样本，检测并分析包括核因子κB（NF-κB）、肿瘤坏死因子α（TNF-α）、鞘氨醇激酶（SphK）、巨噬细胞炎症蛋白1α（MIP-1α）、B细胞淋巴瘤2（BCL2）及Bcl-2相关X蛋白（BAX）在内的多种靶标的基因与蛋白表达水平，同时测定半胱天冬氨酸蛋白酶3/8/9（caspase-3/8/9）的酶活性。 ### 动物与实验方案 本研究共选用36只体质量为180~200g的Sprague-Dawley（SD）大鼠，随机分为3组，每组12只。 1. 对照组：大鼠经乙醚轻度麻醉后，采用润滑的儿科软导管向结肠内注入2ml生理盐水，保持大鼠水平卧位2分钟以防止生理盐水流出。随后每日经口给予由去离子水配制的0.5%（w/v）甲基纤维素与2%（v/v）吐温80（Tween 80）混合溶液，连续干预2周。 2. 溃疡性结肠炎模型组：大鼠经乙醚麻醉后，通过儿科轻柔导管向结肠内注入2ml 4%乙酸，保持水平卧位2分钟以促进乙酸在结肠内滞留。 3. 染料木黄酮干预溃疡性结肠炎组：UC造模…… # 表没食子儿茶素没食子酸酯通过调控炎症与凋亡通路改善大鼠实验性溃疡性结肠炎的治疗效应 [https://doi.org/10.5061/dryad.n02v6wx6v](https://doi.org/10.5061/dryad.n02v6wx6v) ## 数据与文件结构说明 上传的数据为本次实验中不同分组大鼠结肠组织的检测结果，数据通过如下表格进行说明： | 变量名 | 单位 | 描述 | | :---------------------- | :------------------ | :-------------------------------------------------------------------------------------------------------------------------------------------------------------------------- | | 分组 | 无 | 实验组名称。本研究共设置3个实验组：对照组、溃疡性结肠炎模型组……