Whole-methylome and transcriptome of Porphyromonas gingivalis W50

DNA methylation has important roles in bacteria, including in the regulation of transcription. Porphyromonas gingivalis, an oral pathogen in periodontitis, exhibits well-established gene expression changes in response to hemin availability. However, the regulatory processes underlying these effects remain unknown. We profiled the novel P. gingivalis epigenome, and assessed epigenetic and transcriptome variation under limited and excess hemin conditions. For this, P. gingivalis W50 was grown in chemostat continuous culture with excess (ExH, 5 mg/L) or limited hemin (LiH, 0.2 mg/L), prior to whole-methylome and transcriptome profiling using Nanopore and Illumina RNA-Seq. Three independent biological replicates were profiled per experimental condition.