Respiration rate stimulated by endogenous ATPases and inhibited by PK recorded on NKA inhibited cells.

Respiration rate was stimulated by endogenous ATPases and inhibited by endogenous PK (VO2 (PEP)) only or together with exogenously added 20 U/ml PK (VO2 (PEP+PK)). The experimental data are compared with the simulation results obtained by the full models 1, 2, 3, 4 and their corresponding simplified versions (2s, 3s, and 4s). Endogenous ATPases were stimulated by 2 mM ATP and PK was activated by 5 mM PEP added to solution. Shown in bold the optimization result that differs from the experimental value more than two standard deviations.