Near-complete sequence of a novel reovirus genome identified from Callinectes sapidus

The Atlantic blue crab, Callinectes sapidus, is an estuarine keystone species that functions as both predator and prey in food webs and supports a multi-million dollar fishery along the western Atlantic coast from the US mid-Atlantic to southern Brazil. Throughout their range, blue crabs are host to viral, bacterial, fungal, protozoan, and metazoan pathogens. Reoviruses are non-enveloped icosahedral viruses with genomes comprised of 9 to 12 segments of linear double-stranded RNA (dsRNA). They have been found in diverse host species including brachyuran crustaceans. This work describes the sequence of a novel reovirus genome, discovered in Callinectes sapidus. The double-stranded RNA genome of CsRV2 consists of 12 segments that encode 13 putative proteins. High nucleotide sequence identity with Eriocheir sinensis Reovirus 905 revealed this virus belongs to Cardoreovirus within the Reoviridae family.大西洋蓝蟹（Callinectes sapidus）是一类河口关键物种，在食物网中同时充当捕食者与被捕食者，支撑着从美国中大西洋沿岸延伸至巴西南部的西大西洋沿岸价值数百万美元的渔业产业。在其分布范围内，大西洋蓝蟹可作为病毒、细菌、真菌、原生动物及后生动物病原体的宿主。呼肠孤病毒（Reoviruses）为无包膜二十面体病毒，其基因组由9至12个线性双链RNA（double-stranded RNA, dsRNA）片段组成。该类病毒已在包括短尾十足目甲壳动物在内的多种宿主物种中被发现。本研究描述了在大西洋蓝蟹（Callinectes sapidus）中发现的一株新型呼肠孤病毒的基因组序列。CsRV2的双链RNA基因组包含12个片段，可编码13种推定蛋白。通过与中华绒螯蟹呼肠孤病毒905（Eriocheir sinensis Reovirus 905）的核苷酸序列具有高度同源性，确定该病毒隶属于呼肠孤病毒科（Reoviridae）下的心呼肠孤病毒属（Cardoreovirus）。