Comment on “Ancient origins of allosteric activation in a Ser-Thr kinase”

Hadzipasic et al. used ancestral sequence reconstruction to identify historical sequence substitutions that putatively caused Aurora kinases to evolve allosteric regulation. We show that their results arise from an implausible phylogeny and sparse sequence sampling. Addressing either problem reverses their inferences: allostery and the amino acids that confer it were not gained during the diversification of eukaryotes but were lost in a subgroup of Fungi.哈季帕西奇（Hadzipasic）等人借助祖先序列重建（ancestral sequence reconstruction）技术，鉴定出了推定引发极光激酶（Aurora kinases）演化出变构调控（allosteric regulation）的历史序列替换事件。本研究表明，其研究结果源于一个不合理的系统发育树（phylogeny）以及稀疏的序列采样。针对任一问题进行修正，均可推翻其原有推论：变构调控特性及其赋予该特性的氨基酸，并非在真核生物（eukaryotes）的多样化进程中获得，而是在真菌（Fungi）的一个亚群中发生了丢失。